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This paper is intended to investigate the effect of using LTS English Deluxe 10 media of CALL software in teaching listening. The alternate hypothesis in this study was significant differences of listening score before and after treatment. The second-grade students of SMPN 19 Percontohan Banda Aceh, as many as 132 students,were used as the population in this study. The sampleswere the students of the experimental class, taught by using CALL software LTS English Deluxe 10 media, as many as 35 students, and control class, taught bymeans ofconvetional audio,also 35 students.The data were collected by distributing a set of listening test derived from CALL software LTS English Deluxe 10 (2007). Then, the data were analyzed by using Statistical Package for Social Sciences (SPSS) Version 20. The findings showedthat the mean score of the experimental class andincreased significantlyand the control class’s mean score only raised slightlyafter the treatment process. Furthermore, the result of t-test revealed that there was a significant difference between the mean scores of the experimental class and the control class. The findingsattest that the students taught by using CALL software LTS English Deluxe 10 media achieved a better score in listening significantly than those taught by using MP3 player. As a result, the alternate hypothesis was accepted, and the null hypothesis was rejected.